"After close review of recent Tweets from the @realDonaldTrump account and the context around them — specifically how they are being received and interpreted on and off Twitter — we have permanently suspended the account due to the risk of further incitement of violence," the statement reads.
Mr Trump was locked out of his account for 12 hours on Wednesday after he called the people who stormed the US Capitol "patriots".
Hundreds of his supporters entered the complex as the US Congress attempted to certify Joe Biden's victory in the presidential election. The ensuing violence led to the deaths of four civilians and a police officer.
Twitter warned then that it would ban Mr Trump "permanently" if he breached the platform's rules again.
After being allowed back on Twitter, Mr Trump posted two tweets on Friday that the company cited as the final straws.
In one, he wrote: "The 75,000,000 great American Patriots who voted for me, AMERICA FIRST, and MAKE AMERICA GREAT AGAIN, will have a GIANT VOICE long into the future. They will not be disrespected or treated unfairly in any way, shape or form!!!"
Twitter said this tweet "is being interpreted as further indication that President Trump does not plan to facilitate an 'orderly transition'".
In the next, the president tweeted: "To all of those who have asked, I will not be going to the Inauguration on January 20th."
Twitter said this was "being received by a number of his supporters as further confirmation that the election was not legitimate".
As we reported earlier, on January 6, social networks Twitter and Facebook temporarily blocked the accounts of US President Donald Trump, and YouTube deleted one of his videos after crowds of his supporters breached the US Capitol on Wednesday.