The Pentagon said that the strikes were aimed at facilities located a "border control point" used by Iran-backed groups like the Kait'ib Hezbollah (KH) and Kait'ib Sayyid al-Shuhada (KSS).
US officials did not initially reveal if there were any casualties from the strike in Syria. However, the Britain-based Syrian Observatory for Human Rights said that 17 pro-Iran fighters were killed in the attack.
The Pentagon said that Washington had consulted with coalition partners before the move and the operation sent out an unambiguous message that Biden would act to "protect American and Coalition personnel".
"We have acted in a deliberate manner that aims to de-escalate the overall situation in both eastern Syria and Iraq," the statement reads.
The airstrikes were carried out in response to recent attacks on US military and diplomatic sites in Iraq.
In mid-February, over a dozen rockets were fired at a US military base housed at Irbil International Airport in the Kurdish-run region of northern Iraq.
The attack had killed one foreign civilian contractor and wounded a US service member and some coalition troops.
As we reported earlier, two UN experts stated that Iran committed numerous violations of human rights by downing PS752 flight of Ukraine International Airlines and after the deadly crash.
“The inconsistencies in the official explanations seem designed to create a maximum of confusion and a minimum of clarity. They seem contrived to mislead and bewilder," Special Rapporteur on extrajudicial, summary or arbitrary executions Agnes Callamard stated in a 45-page official letter to Iran.